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Get an answer for 'Which developing solvent solution provided the best resolution of the indicator dye? Which one was the worst? Explain. The distance traveled by all solvents was 5cm. First is ...
Would you need to use a more or less polar solvent system to get compound A to move further up the plate? (2 pt) Circle all that apply when performing TLC analysis. (5 pts – no partial credit) Spots should be below the solvent level Spots should be above the solvent level
The result was at least 90 hexane 10 ehtyl acetate. This solvent system gave the best separation because it did not clump all at the top of the solvent front or at the bottom of the spotting line on the TLC plate. Explain. (7 Pts) One of the solvent systems clumped and was very near the solvent front (100% hexane) with Rf value very close to 1.
Label each plate with the appropriate solvent system. Include R f values for each plate. (3 Pts) 3. (a) Which solvent system gave the best results? Explain (7 Pts) (b) What was wrong with the other solvent systems? Explain. (7 Pts) 4. Why was hexane used as a spotting solvent for the extracted pigment? (4 Pts)
Organic Lab-Exam 1. Acetanilide: the 40 mL flask was more pure as indicated by the m.p. because, although it had lower yield, there was less likelihood of collision with impurities in solution TCA: the ethanol and water mixture; allowed tca to stay out of solution because insolubility in water, purity was determined by a more accurate m.p. to that of the literature.
Popular Answers ( 2) You may go for Non-Polar to Polar combination of solvent system which gives you a perfect RF ( .2 to .3 between two spots) and maximum number of spots You may go for this If there are no particular class of compound. If there are particular class of compound then above suggestion given by Frederick Lia is right way.
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stationary phase. The mobile phase is an organic solvent or solvent mixture. The sample mixture is applied near the bottom of the plate as a small spot, then placed in a jar containing a few ml of solvent. The solvent climbs up the plate by capillary action, carrying the sample mixture along with it.
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Solvent Systems for Flash Column Chromatography. Ethyl Acetate/Hexane: The standard, good for ordinary compounds and best for difficult separations. Methanol/Dichloromethane: For polar compounds. 10 percent Ammonia in Methanol Solution/Dichloromethane: Sometimes moves stubborn amines off the baseline.
(a) Which solvent system gave the best results? Explain (7 Pts) The 75:25 hexane: ethyl acetate solvent system gave the best results because the concentration of each compound provided the correct amount of polarity in the solvent to allow the four different pigments to separate out and travel up the plate based on their own polarity.
What solvent system is best for separation of polar compounds (small organic compounds) on a TLC ? ... Its showing good results in the assays so i am trying to separate it further from impurities ...
3a. Which solvent system gave the best results? 3b. What was wrong with the other solvent system? 4. Look up and name four pigments found in spinach. Provide references for your sources. The four pigments found in spinach include carotenes, xanthophylls, chlorophyll a, and chlorophyll b (Juanita College). Part II-Separation of Four Organic Compounds 5a. List all the solvent systems that you tried.
3. Pour approx. 3 mL of solvent into a screw-cap jar, place a piece of filter paper in the jar and wet the paper with the solvent to saturate the atmosphere. Make sure that the solvent is shallow enough that it will be below the spot line on your plate. 4. Place the plate carefully in the chamber (use tongs or tweezers), being careful not to ...
The polarity, dipole moment, polarizability and hydrogen bonding of a solvent determines what type of compounds it is able to dissolve and with what other solvents or liquid compounds it is miscible. Generally, polar solvents dissolve polar compounds best and non-polar solvents dissolve non-polar compounds best: "like dissolves like".
1. Eluent = The solvent that the TLC is placed into inside the chamber. -AKA developing solvent; creates the solvent front, is the mobile phase that rises via cap. action.-In column chromatography, the eluent does the same thing, but moves because of gravity 2. Mobile phase (column) = solvent systems that passes through the stationary phase
Which solvent system, cyclohexane/water, pentane/water, or diethyl ether/water, would give the most efficient extraction into the organic solvent? Pentane/water would be the most efficient extraction system, since it has the highest partition coefficient.
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(c) State your original solvent system and explain your rational for the other Chem 2545 Fall 2007 solvent systems you tried. Explain what observations led you to your best solvent system. 7. Use a drawing to illustrate how triphenylmethanol and benzophenone interact with silica gel. V. Calculations 8. Calculate the R f values of the plate that gave the best separation in Part II, Question 5.
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If two solvent systems give similarly good separation, the cheapest of the non-halogenated solvents should be used. Typically, this will result in a hexanes:ethyl acetate mixture. If you are unsure of the relative cost of a solvent, a good rule of thumb is to look at the size of the container.
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Thin Layer Chromatography Technique. • Performed on glass or plastic plate spread with thin layer of dry adsorbent (solid phase) • Sample spotted on plate using ﬁne capillary tube • Plate put into developing chamber; capillary action draws solvent (mobile phase) up the plate carrying various components with it.
Can anyone please explain how to choose solvent fraction for TLC? ... This is a stronger solvent system and can move more polar compounds. ... best of luck! 2nd Feb, 2018.
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